QuEChERS method and pre-mixesWithin a few years after its devleopment by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowing rapid and cheap clean-up of strong matrix-contaminated samples.Standard clean-up of food samples10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO4 and 1 g NaCI and afterwards centrifuged. 1 ml of the supernantant is spiked with 25 mg CHROMABOND® Diamino and 150 mg MgSO4 and shaken again. After centrifugation the supernatant is injected into the GC/MS.Empty PP centrifuge tube with screw cap, 50 ml, without QuEchERS mixtures.
Octadecyl modified silica phase for SPE, not endcappedBase material silica, pore size 60 Å, particle size 45 µm for C18, specific surface 500 m²/g, pH stability 2 to 8 octadecyl phases, not endcapped, carbon content 14 % possesses more free silanols (SiOH), which allow secondary interactions with polar groups of the analytes.Recommended applications: non-polar compounds, pesticides.
Octadecyl modified silica phase for SPE, endcappedBase material silica, pore size 60 Å, particle size 45 µm for C18 ec, 100 µm for C18 ec f (for fast flow), specific surface 500 m²/g, pH stability 2 to 8. Octadecyl phases, endcapped, carbon content 14 % very non-polar, hydrophobic interactions with a wide variety of organic compounds. Advantageous for clean-up of samples with large structural variations (polarity differences).Recommended applications: non-polar compounds, aflatoxins, amphetamines, antibiotics, antiepileptics, barbiturates, caffeine, drugs, preservatives, fatty acids, nicotine, PAHs, pesticides, PCBs, heavy metals, vitamins. Very well suited for desalting of samples.BIGpacks: 4.003 446 / 4.003 450 / 4.003 452
Octadecyl modified silica phase for SPE of polar analytesBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, special octadecyl phase for polar analytes, not endcapped, carbon content 15%.Recommended applications: more polar compounds like pesticides and their polar degradation products, phenols, phenoxycarboxylic acids, nitroaromatics, pharmaceuticals.
Phenyl modified silica phase for SPEBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8. Phenyl phase, carbon content 8%polarity similar to C8. In addition to hydrophobic interactions more selective adsorption is possible by Π-Π interactions due to the electron density of the phenyl ring.Recommended applications: aflatoxins, caffeine, phenols.
Octyl modified silica phase for SPE, not endcappedBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8. Octyl phase, not endcapped, carbon content 8%, similar to C18, however slightly more polar. Secondary interactions with polar compounds are more pronounced due to shorter alkyl chains.Recommended applications: pesticides, PCBs.
Cyanopropyl modified silica phase for SPEBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2-8, cyanopropyl phase, carbon content 5.5%, polar to mid-polar. In addition to weak hydrophobic interactions selective interactions are possible due to the high electron density of the CN group.Recommended application: cyclosporins, carbohydrates.
Combination phase for SPE analysis of PAHSpecial combination phase, cyanopropyl phase for selective adsorption of polycyclic aromatics via Π-Π interactions, unmodified silica phase for removal of polar compounds.Recommended applications: Extraction of the 16 PAHs according to EPA from soil samples.
Special silica phase for SPE enrichment of drugs from urine or plasmaBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, special bifunctional modification - C8/SA (strong cation exchanger - benzenesulphonic acid).Recommended application: enrichment of acidic, neutral and basic drugs from urine or plasma.
Special silica phase for SPE of THC and derivatives acidic analytes from biological fluids (urine, blood ...)base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, special bifunctional modification - C8/ SB (strong anion exchanger - quaternary amine).Recommended applications: extraction of THC and derivatives from urine, blood, serum, plasma, acidic analytes from biological fluids.
Polar modified polystyrene-divinylbenzene copolymer with a weak anion exchangerSpecific surface 650 to 700 m²/g, particle size 80µm, pore size 50 Å, pH stability 1 to 14. Due to bifunctional modification much more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water.Recommended applications: polar herbicides / pesticides from water (acidic, neutral, basic), polar phenols from water, polyaromatic compounds, polychlorinated biphenyls, drug analysis from urine, blood, serum, plasma pharmaceuticals / active ingredients from tablets, creams.Due to the bifunctional modification CHROMABOND® Easy is considerably more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water. The Easy effect: Aqueous samples can be loaded directly without preconditioning! This means that little or even no conditioning is needed, in contrast to standard SPE materials, where recovery rates normally decrease, in the worst case down to zero! Depending on the separation task conditioning may be required and is recommended for method development. A positive side effect of the excellent wettability: there is no decrease of recovery rates, if the cartridge runs dry, therefore automation is easier or, in some cases compared to silica materials, only feasible with CHROMABOND® Easy, because a permanent vacuum can be used without supervision.Further advantages of using a polymeric material:high surface, this means very high binding capacity (2 - 5 times higher than silica-based adsorbents)less adsorbent is needed in the cartridge (without losing sensitivity or recovery)faster analysis, because the height of the adsorbent bed can be reducedacidic or basic solvents (e. g. TFA) do not destroy the phase, or lead to unintended side productsBecause of the polar modification the material is suitable for a broad range of compounds (acidic, neutral, basic, polar and nonpolar substances). Highly reproducible recovery rates can be obtained, even if the cartridge runs dry (especially advantageous when using 96-well plates, where stopcocks are not available!)BIGpacks: 4.003 723 / 4.003 724
Polystyrene-divinylbenzene adsorbent resin for SPEBasic material: Highly porous polystyrene-divinylbenzene copolymer specific surface 1200 m2/g.Particle size 50-100 µm very high binding capacity, up to 30 % of adsorbent weight.Recommended application: aromatic compounds, phenols from water, nitroaromatics from water, pesticides from water, PAHs from oil*Glass column
Spherical, hydrophobic polystyrene-divinylbenzene resin for SPEHydrophobic polystyrene-divinylbenzene copolymer pH stability 1 to14. High-purity material with highest reproducibility and lowest blank values due to a novel manufacturing process, spherical particles 85mm; pore size 55 to 60Å. Very high surface 1000m²/g; capacity 390mg/g (caffeine in water). Excellent recovery rates especially for the enrichment of pharmaceuticals/active ingredients due to the spherical structure of the particles, very homogeneous surface, and optimised pore structure.Recommended applications: pharmaceuticals/active ingredients from tablets, creams and water/waste water, drugs and pharmaceuticals from urine, blood, serum and plasma trace analysis of pesticides.Cartridges in further sizes and phases of the HR-X poduct range available on request.
The innovative cation exchanger offers:mixed-mode SPE phase with distinct cation exchange and RP properties excellent enrichment of basic analytesmodern support polymer with optimised pore structure and large specific surfacegood reproducibility, reliable and cost-efficient analysesmore aggressive washing procedures for matrix removal possiblecleaner samples and protection of analytical HPLC and GC instrumentationquantificationof analytes from heavily contaminated sampleslower limits of detection also for critical matrices... the perfect fit for basic analytes in comination with CHROMABOND® HR-XBenzenesulfonic acid modified polymeric cation exchanger for SPE Base material spherical PS/DVB copolymer, pore size 65-75Å, particle sizes 45µm or 85µm (standard), very large specific surface 800m²/g, pore volume 1.4cm³/g, pH stability 1-14. Benzenesulfonic acid modification, exchange capacity 1.0meq/g, pKa < 1 RP capacity 300mg/g (caffeine in water). High-purity material, highest reproducibility and lowest blank values due to an optimized production process, outstanding recovery rates especially for the enrichment of basic analytes for standard protocol.Recommended application: basic active ingredients from strongly matrix-contaminated samples like, e.g., urine, plasma, serum, fungicides from food, basic analytes like, e.g., amines, bases with pKa 2-10.
Combination phase for SPE of hydrocarbons from water acc. to DIN H53 / ISO DIS 9377-4Special combination phase of sodium sulphate and Florisil®.Recommended application: hydrocarbons from drinking, surface and waste waters.*Glass columns
Special phase for SPE analysis of PCB from sludgeSpecial combination phase:N: sodium sulphate for removal of trace water.A: SiOH/AgNO3 phase for removal of sulphur, sulphur-containing and polar compounds.Recommended application: extraction of PCB from sludge.BIGpacks: 4.003 517
Combination phase for SPE analysis of PAH from water containing humic acidsSpecial combination phase: aminopropyl phase for removal of interfering humic acids, octadecyl phase for enrichment of PAH.Recommended application: PAH from water containing humic acids.Glass columns available on request.
The innovative cation exchanger offers:mixed-mode SPE phase with distinct cation exchange and RP properties excellent enrichment of basic analytesmodern support polymer with optimised pore structure and large specific surfacegood reproducibility, reliable and cost-efficient analysesmore aggressive washing procedures for matrix removal possiblecleaner samples and protection of analytical HPLC and GC instrumentationquantificationof analytes from heavily contaminated sampleslower limits of detection also for critical matrices... the perfect fit for basic analytes in comination with CHROMABOND® HR-XSPE phases for polymer-based RP and ion chromatographyBase material: high-purity polystyrene-divinylbenzene copolymers (PS/DVB) pore size 100 Å, paricle size 100µm. Verly low degree of swelling, thus very well suited for chromatography. Different modifications for different applications from elimination of nonpolar compounds up to removal of specific polar comonents.Recommended application:Removal of interfering compounds improves chromatographic separtions, if the interfering components overlap with the analyte in the chromatogramimproves lifetime of the chromatographic column, since interfering components can irreversibly block the column packingremoval or concentration of anions from water increasing the pH value in acidic samples removal or concentration of cations from waterdecreasing the pH value in basic samplesPS-H+ strong PS/DVB cation exchanger, H+ form, capacity 2,9 meg/g